Journal:

Article Title: Polyamine Composition and Expression of Genes Related to Polyamine Biosynthesis in an Aphid Endosymbiont, Buchnera

doi:

Figure Lengend Snippet: Relative amounts of speD and speE mRNAs analyzed by quantitative RT-PCR. Total RNA was prepared from bacteriocytes of aphids at the indicated ages (days). cDNAs were synthesized with pd(N)6 primer using the First-Strand cDNA synthesis kit (Pharmacia) and quantified by PCR amplification with a LightCycler instrument (Boehringer Mannheim). Amounts of target cDNAs in each sample are expressed as a proportion of that in Buchnera from 10-day-old aphids. Each data point is the mean ± standard error of five replicate groups. (A) Relative amount of 16S rRNA. The value of each data point was used for calibration in panels B and C. (B) Relative amount of speD mRNA. (C) Relative amount of speE mRNA.

Article Snippet: A LightCycler (Boehringer Mannheim) instrument was used for real-time quantitative PCR.

Techniques: Quantitative RT-PCR, Synthesized, Amplification